Laser in Inflammation
There are several studies that show the effectiveness of low power laser radiation in reducing some types of pain, treating inflammation and wound healing. However, more evidence is required to prove the effectiveness of low power laser therapy (LLLT) and many aspects of the cellular and molecular mechanisms, triggered by irradiation of tissue with laser, remain unknown.
Considering these facts, we intend to determine the influence of laser irradiation parameters, including wavelength, dose, power and time of interaction on the expression of anti-inflammatory cytokines (IL-2, IL-6) and pro-inflammatory cytokines (IL-1b, TNF-a) in the injured region and assess the clinical consequences of this cellular level effect.
We will begin by evaluating the effect of wavelength and dose delivered under conditions of continuous illumination in the treatment of inflammation induced in the gastrocnemius muscle of Wistar rats, by quantification of cytokines and histological and immunohistochemical analysis of muscle tissue. In a second phase, we will evaluate the more adequate radiant power for inflammation treatment. The work will end with a comparative assessment of continuous and pulsed laser irradiation. This approach will develop and improve the technique of low-power laser therapy by determining the best therapeutic parameters, based on the quantification of cellular markers.
This project was concluded in 2014.